FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304375
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC100590.7711739338763777Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT63630.4878198370867274TruSeq Adapter, Index 22 (97% over 37bp)
TTACCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA55380.42457115476760904TruSeq Adapter, Index 22 (97% over 37bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG42660.32705318639195013TruSeq Adapter, Index 22 (97% over 37bp)
TTACCTTGACTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38140.2924005749880211Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT24760.1898227120268328TruSeq Adapter, Index 22 (97% over 37bp)
TTACCTTGACTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC21510.16490656444657403TruSeq Adapter, Index 11 (97% over 36bp)
TTACCTTGACTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG17850.1368471490177288TruSeq Adapter, Index 2 (100% over 35bp)
TTACCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGAT16450.1261140392908481TruSeq Adapter, Index 22 (97% over 37bp)
TTACCTTGACTTACCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA14170.10863440344992811Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)