FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408273
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAATTCTATTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT105770.43919439365885843Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAATTCTATTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT43150.17917403882367158TruSeq Adapter, Index 1 (97% over 35bp)
TCAATTCTATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCC31070.12901361265936212TruSeq Adapter, Index 18 (97% over 37bp)
TCAATTCTATTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC27690.11497865898093779TruSeq Adapter, Index 1 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)