FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586820
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG95700.36995229664220936TruSeq Adapter, Index 15 (97% over 36bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAA71060.27470021106996234TruSeq Adapter, Index 25 (97% over 38bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC65210.25208557224700595TruSeq Adapter, Index 5 (100% over 35bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG51730.19997525919855266TruSeq Adapter, Index 5 (97% over 37bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA37180.14372859340812272Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAATC34230.13232463024099086TruSeq Adapter, Index 25 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)