FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240272
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG153971.2414212366319646TruSeq Adapter, Index 5 (97% over 37bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG152731.2314234296992919TruSeq Adapter, Index 15 (97% over 36bp)
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA121480.9794625695008836Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC60060.4842486164325244TruSeq Adapter, Index 5 (100% over 35bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA34310.2766328676290362TruSeq Adapter, Index 25 (97% over 38bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA27500.22172555697459911TruSeq Adapter, Index 25 (97% over 38bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAA17130.1381148651263594TruSeq Adapter, Index 25 (97% over 38bp)
GCTTGCCATTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG12430.10021995175251881TruSeq Adapter, Index 15 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)