FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005713125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315700
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG173751.3205898001064074TruSeq Adapter, Index 15 (97% over 36bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG122630.9320513794938056TruSeq Adapter, Index 5 (97% over 37bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA84690.6436877707684123TruSeq Adapter, Index 25 (97% over 38bp)
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC84620.6431557345899521Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA55000.41802842593296347Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC48520.36877707684122524TruSeq Adapter, Index 5 (100% over 35bp)
GAGGCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG48340.36740898381089915TruSeq Adapter, Index 15 (97% over 36bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA45410.34513946948392493TruSeq Adapter, Index 25 (97% over 38bp)
GAGGCTTGGTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC35350.26867827012236833TruSeq Adapter, Index 5 (100% over 35bp)
GAGGCTTGGTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC32490.2469407919738542TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAA29730.2259633655088546TruSeq Adapter, Index 25 (97% over 38bp)
GAGGCTTGGTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC28950.22003496237744166Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA23710.18020825416128297Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC19830.1507182488409212TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAT18550.14098958729193586TruSeq Adapter, Index 25 (97% over 38bp)
GGTCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACA15020.11415976286387475TruSeq Adapter, Index 25 (97% over 38bp)
GAGGCTTGGTTTAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA13600.10336702895796913Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)