FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335565
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG177871.331795906601326TruSeq Adapter, Index 15 (97% over 36bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG124230.9301681310905872TruSeq Adapter, Index 5 (97% over 37bp)
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC89100.6671333854960261Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA86760.6496127107254234TruSeq Adapter, Index 25 (97% over 38bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA57250.42865753445171145Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC51440.3851553462392321TruSeq Adapter, Index 5 (100% over 35bp)
GAGGCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG49650.3717527787865061TruSeq Adapter, Index 15 (97% over 36bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA43250.3238329845421226TruSeq Adapter, Index 25 (97% over 38bp)
GAGGCTTGGTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC37210.2786086787239857TruSeq Adapter, Index 5 (100% over 35bp)
GAGGCTTGGTTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC32450.24296833175472551TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAA30650.2294908896234927TruSeq Adapter, Index 25 (97% over 38bp)
GAGGCTTGGTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC30480.22821802008887626Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA23940.17924998034539688Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC21330.15970768925510925TruSeq Adapter, Index 5 (100% over 35bp)
GGTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAT18570.13904227798721888TruSeq Adapter, Index 25 (97% over 38bp)
GGTCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACA15720.11770299461276688TruSeq Adapter, Index 25 (97% over 38bp)
GAGGCTTGGTTTAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA13530.1013054400197669Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)