FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890351
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCC181102.034029276094484TruSeq Adapter, Index 18 (97% over 37bp)
TAGCCTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC132441.487503243103001TruSeq Adapter, Index 1 (97% over 36bp)
TAGCCTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCA57830.6495191222338157TruSeq Adapter, Index 18 (97% over 37bp)
TAGCCTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAA40590.45588762184801274TruSeq Adapter, Index 18 (97% over 37bp)
TAGCCTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC37310.41904821806231474Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCCTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC36110.4055703874090106TruSeq Adapter, Index 1 (97% over 36bp)
TAGCCTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT34580.3883861533260478Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCCTGGTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCC10720.12040195383618371TruSeq Adapter, Index 18 (97% over 37bp)
TAGCCTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC10300.11568471310752725Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCCTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT10270.11534776734119466TruSeq Adapter, Index 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)