FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047591
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC140481.340981356273584Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG73660.7031370067134979TruSeq Adapter, Index 12 (100% over 37bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT60410.5766563477540376TruSeq Adapter, Index 12 (100% over 38bp)
TTACCTTGACTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC47140.44998477459237435Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT23880.22795155743033305TruSeq Adapter, Index 12 (100% over 36bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTC23810.22728335772262265TruSeq Adapter, Index 12 (97% over 38bp)
TTACCTTGACTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT21790.20800102330012382TruSeq Adapter, Index 1 (97% over 35bp)
TTACCTTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT16990.16218161477141366TruSeq Adapter, Index 12 (100% over 38bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC15950.15225407625685977TruSeq Adapter, Index 12 (97% over 38bp)
TTACCTTGACTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC15790.15072676263923612Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC14120.13478542675528904TruSeq Adapter, Index 12 (97% over 38bp)
TTACCTTGACTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13430.12819888677928695Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTTACCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA13260.1265761160605618Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC12990.12399877433082185TruSeq Adapter, Index 12 (97% over 38bp)
TTACCTTGACTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC11880.11340303610855765Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC11850.11311666480525319TruSeq Adapter, Index 12 (97% over 38bp)
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC11650.1112075227832236TruSeq Adapter, Index 12 (97% over 38bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC10480.10003904195435051TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)