FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309622
Sequences flagged as poor quality0
Sequence length10-85
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTACTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58980.45035895853918156Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTACTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA24090.1839462073789231TruSeq Adapter, Index 22 (97% over 37bp)
CTGGTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGAT18880.14416373579551964TruSeq Adapter, Index 22 (97% over 37bp)
CATTACTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG18850.14393466206279368TruSeq Adapter, Index 2 (100% over 35bp)
CATTACTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC17070.13034295392105508Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)