FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601505
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA349412.181760281734993TruSeq Adapter, Index 22 (97% over 37bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC177081.1057099415861955TruSeq Adapter, Index 11 (97% over 36bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC132100.8248491262905829Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAT114440.7145778502096465TruSeq Adapter, Index 22 (97% over 37bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATG94960.5929422636832229TruSeq Adapter, Index 22 (97% over 37bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC84400.5270042865929235Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC46970.2932866272662277TruSeq Adapter, Index 11 (97% over 36bp)
CTGGTTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG24160.15085809910053355TruSeq Adapter, Index 2 (100% over 35bp)
CTGGTTGGTTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC23580.14723650566186183Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC21400.13362430963375077Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG18940.11826375815248782TruSeq Adapter, Index 2 (100% over 35bp)
CTGGTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGA18700.11676516776407192TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)