FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115887
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCATTAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA84230.7548255334097449Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGCCATTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA47410.42486380789452693TruSeq Adapter, Index 25 (97% over 38bp)
GAGCCATTAGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC39510.3540681090468838TruSeq Adapter, Index 5 (100% over 35bp)
GAGCCATTAGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG26930.24133267974266212TruSeq Adapter, Index 15 (97% over 36bp)
GAGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACA25580.22923468057249521TruSeq Adapter, Index 25 (97% over 38bp)
TAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAA20340.1822765208305142TruSeq Adapter, Index 25 (97% over 38bp)
TAGAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACA20090.18003615061381664TruSeq Adapter, Index 25 (97% over 38bp)
GAGCCATTAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG16640.14911904162339018TruSeq Adapter, Index 5 (97% over 37bp)
GAGCCATTAGTTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA15300.1371106572618912Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGCCATTAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14960.13406375376718252Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGCCATTAGTTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC13390.11999422880632178TruSeq Adapter, Index 5 (100% over 35bp)
GAGCCATTAATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC12810.11479656990358343TruSeq Adapter, Index 5 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)