FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221671
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC93960.7691105052014823TruSeq Adapter, Index 11 (97% over 36bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG56380.4614990451602764TruSeq Adapter, Index 2 (100% over 35bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATA55780.45658773925222096TruSeq Adapter, Index 22 (97% over 37bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA45290.3707217409597183TruSeq Adapter, Index 22 (97% over 37bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATATCTCGTATGCCGTCTTCTGCTTGAA29710.24319149754721195TruSeq Adapter, Index 22 (97% over 37bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC28590.23402372651884185Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATATCTCGTATGCCGTCTTCTGCTTGAA16250.1330145350098349TruSeq Adapter, Index 22 (97% over 37bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATATCGCGTATGCCGTCTTCTGCTTGAA13140.10755759938641418TruSeq Adapter, Index 22 (97% over 37bp)
GACGACCAGATTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG13050.10682090350020586TruSeq Adapter, Index 2 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATATCGCGTATGCCGTCTTCTGCTTGAA12630.10338298936456705TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)