FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234535
Sequences flagged as poor quality0
Sequence length10-85
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC98330.7964942265711381TruSeq Adapter, Index 11 (97% over 36bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG56630.4587152247607399TruSeq Adapter, Index 2 (100% over 35bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATA56210.4553131340950236TruSeq Adapter, Index 22 (97% over 37bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA47080.3813581631950491TruSeq Adapter, Index 22 (97% over 37bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATATCTCGTATGCCGTCTTCTGCTTGAA31110.25199771573912444TruSeq Adapter, Index 22 (97% over 37bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC28640.23199018253836465Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATATCTCGTATGCCGTCTTCTGCTTGAA16700.13527360504157437TruSeq Adapter, Index 22 (97% over 37bp)
GACGACCAGATTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG13860.11226899196863596TruSeq Adapter, Index 2 (100% over 35bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATATCGCGTATGCCGTCTTCTGCTTGAA13820.11194498333380586TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)