FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420230
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTCTCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA174741.2303640959562887Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACTCTCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG96210.6774254874210515TruSeq Adapter, Index 5 (97% over 37bp)
TACTCTCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC71800.5055519176471417TruSeq Adapter, Index 5 (100% over 35bp)
TACTCTCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG70720.4979475155432571TruSeq Adapter, Index 15 (97% over 36bp)
TACTCTCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA44930.3163572097477169TruSeq Adapter, Index 25 (97% over 38bp)
TACTCTCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA36010.25355048126007756TruSeq Adapter, Index 25 (97% over 38bp)
TACTCTCATTATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA15330.10794026319680616TruSeq Adapter, Index 5 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)