FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204693
Sequences flagged as poor quality0
Sequence length10-85
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC113850.9450540511150974Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG100990.838304862732663TruSeq Adapter, Index 12 (100% over 37bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT70760.5873695622038145TruSeq Adapter, Index 12 (100% over 36bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT45080.3742032202395133TruSeq Adapter, Index 1 (97% over 35bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT43590.3618349239183759TruSeq Adapter, Index 12 (100% over 38bp)
CATGGCTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC26110.21673571607040135Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC25980.21565660296855713TruSeq Adapter, Index 12 (97% over 38bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC22050.18303418381280542TruSeq Adapter, Index 12 (97% over 38bp)
CATGGCTGGCTTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC21380.17747260090330066Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC18210.15115884295833046TruSeq Adapter, Index 12 (97% over 38bp)
CATGGCTGGCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC17710.14700840795123737Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC17560.14576327744910944TruSeq Adapter, Index 12 (97% over 38bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC16000.132813920226979TruSeq Adapter, Index 12 (97% over 38bp)
CATGGCTGGCTTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC15640.12982560702187196Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC13960.11588014539803917TruSeq Adapter, Index 12 (97% over 38bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCGC13010.10799431888456228TruSeq Adapter, Index 12 (97% over 38bp)
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTC12800.1062511361815832TruSeq Adapter, Index 12 (97% over 38bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTCGGTT12650.10500600567945526TruSeq Adapter, Index 12 (97% over 38bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATC12410.10301379687605058TruSeq Adapter, Index 12 (97% over 38bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCGAATCTCGGTT12090.100357518471511TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)