FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678335
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATACTTCTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA236783.490605674187533Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCATACTTCTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC47790.7045191535155934TruSeq Adapter, Index 5 (100% over 35bp)
TCATACTTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG30090.4435861336950032TruSeq Adapter, Index 5 (97% over 37bp)
TCATACTTCTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG18940.27921307318655236TruSeq Adapter, Index 15 (97% over 36bp)
TCATACTTCTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG12540.18486441065255368TruSeq Adapter, Index 15 (97% over 36bp)
TCATACTTCATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC10160.14977850177272292TruSeq Adapter, Index 5 (97% over 35bp)
TCATACTTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA8690.12810779334694508TruSeq Adapter, Index 25 (97% over 38bp)
TCATACTTCTTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8250.12162132279773268Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)