FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367089
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAATTCTATTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA192111.4052486707156593Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAATTCTATTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC68560.5015035597536078TruSeq Adapter, Index 5 (100% over 35bp)
TCAATTCTATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG55310.4045822912773053TruSeq Adapter, Index 5 (97% over 37bp)
TCAATTCTATTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG50640.37042211589735563TruSeq Adapter, Index 15 (97% over 36bp)
TCAATTCTATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA30260.22134623276172946TruSeq Adapter, Index 25 (97% over 38bp)
TCAATTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACA23290.17036198813683673TruSeq Adapter, Index 25 (97% over 38bp)
TCAATTCTAATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC14350.10496756246301449TruSeq Adapter, Index 5 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)