FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382580
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAATTCTATTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA198701.43716819279897Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAATTCTATTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC71300.5157025271593687TruSeq Adapter, Index 5 (100% over 35bp)
TCAATTCTATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG55860.4040272533958252TruSeq Adapter, Index 5 (97% over 37bp)
TCAATTCTATTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG54440.393756599979748TruSeq Adapter, Index 15 (97% over 36bp)
TCAATTCTATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGA31830.23022175931953306TruSeq Adapter, Index 25 (97% over 38bp)
TCAATTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACA21740.1572422572292381TruSeq Adapter, Index 25 (97% over 38bp)
TCAATTCTAATATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC15400.11138595958280896TruSeq Adapter, Index 5 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)