FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709254
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATACTTCTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT152402.1487365598220105Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCATACTTCTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT33630.47416017392922705TruSeq Adapter, Index 1 (97% over 35bp)
TCATACTTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCC18410.2595685043721995TruSeq Adapter, Index 18 (97% over 37bp)
TCATACTTCTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC10110.1425441379252003TruSeq Adapter, Index 1 (97% over 36bp)
TCATACTTCATATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCAAG8150.11490946825819806TruSeq Adapter, Index 3 (97% over 35bp)
TCATACTTCTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTC7570.10673186192816679TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)