FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23466126
Sequences flagged as poor quality0
Sequence length10-85
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA1062300.4526950890828763TruSeq Adapter, Index 7 (97% over 37bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC731170.311585303854586Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG553820.23600827848618897TruSeq Adapter, Index 7 (97% over 36bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAC442140.18841627288628723TruSeq Adapter, Index 7 (97% over 37bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG439030.18709095826043037Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACG380850.16229777339472226TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)