FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5360656
Sequences flagged as poor quality0
Sequence length10-85
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA338380.6312287152915613TruSeq Adapter, Index 7 (97% over 37bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACG234920.4382299479765163TruSeq Adapter, Index 7 (97% over 37bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC228260.4258060953734021Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG216480.403831172901227TruSeq Adapter, Index 7 (97% over 36bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAC167500.3124617584116571TruSeq Adapter, Index 7 (97% over 37bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACGA107630.20077766601699493TruSeq Adapter, Index 7 (97% over 37bp)
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG71050.13253974886655662Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTATATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACGA63720.11886604922979577TruSeq Adapter, Index 9 (97% over 35bp)
GGTCTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG56390.10519234959303489TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)