FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5448954
Sequences flagged as poor quality0
Sequence length10-85
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA351340.6447843017210275TruSeq Adapter, Index 7 (97% over 37bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACG237020.4349825672964022TruSeq Adapter, Index 7 (97% over 37bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC236230.43353274775305495Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG224700.4123727232786329TruSeq Adapter, Index 7 (97% over 36bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAC170820.31349135999312894TruSeq Adapter, Index 7 (97% over 37bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACGA103370.1897061344250658TruSeq Adapter, Index 7 (97% over 37bp)
GGTCTTGGTATATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACGA73350.1346129917778715TruSeq Adapter, Index 9 (97% over 35bp)
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG72410.13288789004274948Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG57530.1055798966186905TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)