FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6405490
Sequences flagged as poor quality0
Sequence length10-85
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA574310.896590268660165TruSeq Adapter, Index 7 (97% over 37bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG404560.6315832200190774TruSeq Adapter, Index 7 (97% over 36bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC329910.5150425650496684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC203840.31822702088364824Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAT181150.2828042819518881TruSeq Adapter, Index 7 (97% over 38bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATC148200.23136403304040754TruSeq Adapter, Index 7 (97% over 39bp)
CTGGTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG107330.16755939046037072TruSeq Adapter, Index 7 (97% over 36bp)
CTGGTTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT66250.10342690410881915TruSeq Adapter, Index 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)