FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6493494
Sequences flagged as poor quality0
Sequence length10-85
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA591850.91145075363125TruSeq Adapter, Index 7 (97% over 37bp)
CTGGTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG417670.6432130375418842TruSeq Adapter, Index 7 (97% over 36bp)
CTGGTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC337530.519797200089813Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC209190.32215321982279493Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAT189670.2920923619857045TruSeq Adapter, Index 7 (97% over 38bp)
CTGGTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATC148960.2293988413633708TruSeq Adapter, Index 7 (97% over 39bp)
CTGGTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG111930.1723725316447509TruSeq Adapter, Index 7 (97% over 36bp)
CTGGTTGGTTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT66960.10311859840018331TruSeq Adapter, Index 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)