FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14413516
Sequences flagged as poor quality0
Sequence length10-85
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG975670.6769132528107646TruSeq Adapter, Index 7 (97% over 36bp)
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA943670.6547118690540185TruSeq Adapter, Index 7 (97% over 37bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC768040.5328609618916024Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATC507010.35176011182836997TruSeq Adapter, Index 7 (97% over 39bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAT441130.306053013019169TruSeq Adapter, Index 7 (97% over 38bp)
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC226160.15690827970080307Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCG206180.1430462907176847TruSeq Adapter, Index 7 (97% over 39bp)
GGTCTTGGTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG200240.1389251588578387TruSeq Adapter, Index 7 (97% over 36bp)
GGTCTTGGTTTTAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT191890.13313198528381276TruSeq Adapter, Index 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)