FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13781161
Sequences flagged as poor quality0
Sequence length10-85
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA375860.27273464115251245Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTC189020.13715825538936816TruSeq Adapter, Index 13 (97% over 37bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT184080.13357365174095276TruSeq Adapter, Index 8 (100% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)