FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12018227
Sequences flagged as poor quality0
Sequence length10-85
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG365790.3043626984246512Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA194000.16142148088898636TruSeq Adapter, Index 7 (97% over 37bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAC170470.14184288581002838TruSeq Adapter, Index 7 (97% over 37bp)
CATGGCTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC166720.13872262522583406Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG165060.13734138987389735TruSeq Adapter, Index 7 (97% over 36bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA140420.11683919766201786TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)