FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12223486
Sequences flagged as poor quality0
Sequence length10-85
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG380190.31103238470596684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA197440.16152511648477366TruSeq Adapter, Index 7 (97% over 37bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAC176420.14432871277473544TruSeq Adapter, Index 7 (97% over 37bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG175040.14319973860157406TruSeq Adapter, Index 7 (97% over 36bp)
CATGGCTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC171990.14070454205944194Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA145440.11898406068448886TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)