FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15969289
Sequences flagged as poor quality0
Sequence length10-85
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC331900.2078364290357573Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTC305480.19129217337102486TruSeq Adapter, Index 13 (97% over 37bp)
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT267240.16734621059209337TruSeq Adapter, Index 8 (100% over 36bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCT222270.13918590865253927TruSeq Adapter, Index 13 (97% over 37bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA195020.12212190536472851Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)