FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14070752
Sequences flagged as poor quality0
Sequence length10-85
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT799610.5682780849239615Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA479750.3409554798492646TruSeq Adapter, Index 2 (97% over 36bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAG446610.31740307838557597TruSeq Adapter, Index 3 (97% over 37bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG283300.2013396298932708TruSeq Adapter, Index 2 (97% over 35bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCT283200.20126856048631944TruSeq Adapter, Index 4 (97% over 38bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGC260230.18494391770958654TruSeq Adapter, Index 4 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)