FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31904959
Sequences flagged as poor quality0
Sequence length10-85
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTA1325050.4153116134704953TruSeq Adapter, Index 7 (97% over 39bp)
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG777160.24358595790704513TruSeq Adapter, Index 7 (97% over 36bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT724550.22709635828085536TruSeq Adapter, Index 1 (97% over 35bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA401780.1259302668277994TruSeq Adapter, Index 7 (97% over 37bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC335950.10529711070934146Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTATCTCGTATGCCGTCTTCTGCTTGAA326070.10220041342162514TruSeq Adapter, Index 7 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)