FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32331534
Sequences flagged as poor quality0
Sequence length10-85
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTA1303300.4031049068070819TruSeq Adapter, Index 7 (97% over 39bp)
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG796780.24644051841152975TruSeq Adapter, Index 7 (97% over 36bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT740720.22910140916914118TruSeq Adapter, Index 1 (97% over 35bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA414940.12833910076769015TruSeq Adapter, Index 7 (97% over 37bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC344630.10659252975748074Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTATCTCGTATGCCGTCTTCTGCTTGAA338050.10455736495521678TruSeq Adapter, Index 7 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)