FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16483992
Sequences flagged as poor quality0
Sequence length10-85
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1131560.6864599303372629Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA848790.5149177456528734TruSeq Adapter, Index 7 (97% over 37bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG620630.3765046719265576TruSeq Adapter, Index 7 (97% over 36bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA421030.25541749838267336TruSeq Adapter, Index 7 (97% over 35bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACG381290.23130926052378573TruSeq Adapter, Index 7 (97% over 37bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAC313460.19016024759051084TruSeq Adapter, Index 7 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)