FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13369426
Sequences flagged as poor quality0
Sequence length10-85
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC719920.5384823551886222Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA420610.31460587761957765TruSeq Adapter, Index 7 (97% over 37bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAC412760.308734271763051TruSeq Adapter, Index 7 (97% over 37bp)
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG326640.24431864165297745TruSeq Adapter, Index 7 (97% over 36bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACG289790.21675575301437774TruSeq Adapter, Index 7 (97% over 37bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG270600.20240210761479213Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA206950.1547934817844835TruSeq Adapter, Index 7 (97% over 35bp)
GAGGCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG146930.10990000617827572TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)