FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11277921
Sequences flagged as poor quality0
Sequence length10-85
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTTGACTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC437270.3877221697154999Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC240660.21339039349539687Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTACCTTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAT201390.17857014604021432TruSeq Adapter, Index 7 (97% over 38bp)
TTACCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA183080.16233488423974596TruSeq Adapter, Index 7 (97% over 37bp)
TTACCTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATC155710.1380662269224975TruSeq Adapter, Index 7 (97% over 39bp)
TTACCTTGACTTACCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA127180.11276901123886221Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)