FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15068740
Sequences flagged as poor quality0
Sequence length10-85
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCCATTAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG845090.5608232672406585Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGCCATTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAC560830.37218108481531964TruSeq Adapter, Index 7 (97% over 37bp)
GAGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACGAT465150.3086853977173938TruSeq Adapter, Index 7 (97% over 37bp)
GAGCCATTAGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA449730.2984522926269881TruSeq Adapter, Index 7 (97% over 35bp)
GAGCCATTAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC189680.12587648336888155Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)