FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15112838
Sequences flagged as poor quality0
Sequence length10-85
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA407080.2693603941231951TruSeq Adapter, Index 2 (97% over 36bp)
GACGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTAGA375190.24825912909276207TruSeq Adapter, Index 4 (97% over 38bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG359300.23774488947740988TruSeq Adapter, Index 2 (97% over 35bp)
GACGACCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAG245080.16216676179550127TruSeq Adapter, Index 3 (97% over 37bp)
GACGACCAGATTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT176370.11670210452861336Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)