FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17307619
Sequences flagged as poor quality0
Sequence length10-85
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTCTCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG898670.5192337547989703Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACTCTCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA452500.2614455518116039TruSeq Adapter, Index 7 (97% over 35bp)
TACTCTCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA352150.20346530623305262TruSeq Adapter, Index 7 (97% over 37bp)
TACTCTCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACG298410.17241539694165903TruSeq Adapter, Index 7 (97% over 37bp)
TACTCTCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAC290260.16770648810792518TruSeq Adapter, Index 7 (97% over 37bp)
TACTCTCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG195350.11286936695336315TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)