FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17608218
Sequences flagged as poor quality0
Sequence length10-85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTCTCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG930240.5282987750378829Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACTCTCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA463960.26349060421673565TruSeq Adapter, Index 7 (97% over 35bp)
TACTCTCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA370330.21031656922920874TruSeq Adapter, Index 7 (97% over 37bp)
TACTCTCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAC301200.17105649191758077TruSeq Adapter, Index 7 (97% over 37bp)
TACTCTCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACG283080.16076584240381395TruSeq Adapter, Index 7 (97% over 37bp)
TACTCTCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG203710.11569029870030006TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)