FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10779697
Sequences flagged as poor quality0
Sequence length10-85
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGCTGGCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC380660.3531268086663289Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG224970.20869788826160884TruSeq Adapter, Index 7 (97% over 36bp)
CATGGCTGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA188590.17494925877786732TruSeq Adapter, Index 7 (97% over 37bp)
CATGGCTGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAT158330.1468779688334468TruSeq Adapter, Index 7 (97% over 38bp)
CATGGCTGGCTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC152770.14172012441537085Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGGCTGGCTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT145080.13458634319684495TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)