FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005713541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005713541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7286581
Sequences flagged as poor quality0
Sequence length10-85
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATACTTCTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1161871.5945338424152564Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCATACTTCTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA308740.4237103793946709TruSeq Adapter, Index 7 (97% over 35bp)
TCATACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACGA91690.12583405029052722TruSeq Adapter, Index 7 (97% over 37bp)
TCATACTTCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA90060.12359706150250716TruSeq Adapter, Index 7 (97% over 37bp)
TCATACTTCATATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGACGA83480.1145667632048556TruSeq Adapter, Index 9 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)