FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4287369
Sequences flagged as poor quality0
Sequence length10-85
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACCAGATTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG60230.14048242640183292TruSeq Adapter, Index 7 (97% over 36bp)
GACGACCAGATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC50100.11685488232993241TruSeq Adapter, Index 5 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)