FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4055740
Sequences flagged as poor quality0
Sequence length10-85
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC132220.3260070911843461Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC76310.18815308673632924TruSeq Adapter, Index 16 (100% over 38bp)
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG66860.16485277655865516TruSeq Adapter, Index 7 (97% over 36bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC66540.16406377134628947TruSeq Adapter, Index 16 (100% over 39bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT57900.14276063061241598TruSeq Adapter, Index 16 (100% over 37bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC46070.11359209416777209Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)