FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177366
Sequences flagged as poor quality0
Sequence length10-85
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC132610.3174488421651347Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC78250.18731899479241226TruSeq Adapter, Index 16 (100% over 38bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC69140.16551099424852886TruSeq Adapter, Index 16 (100% over 39bp)
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG67970.1627101862752749TruSeq Adapter, Index 7 (97% over 36bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT58750.14063886190484626TruSeq Adapter, Index 16 (100% over 37bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC45470.10884849448192953Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)