FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763000
Sequences flagged as poor quality0
Sequence length10-85
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC69840.39614293817356777Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG56150.3184912081678956TruSeq Adapter, Index 7 (97% over 36bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT51920.29449801474758935TruSeq Adapter, Index 16 (100% over 37bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCA40060.22722631877481564TruSeq Adapter, Index 16 (97% over 39bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC39850.22603516732841747TruSeq Adapter, Index 16 (100% over 39bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC26700.1514463981849121TruSeq Adapter, Index 5 (97% over 35bp)
GCTTGCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG24930.14140669313669882TruSeq Adapter, Index 16 (97% over 39bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC21360.12115711854792967TruSeq Adapter, Index 16 (100% over 38bp)
CATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCAT19620.11128757799205899TruSeq Adapter, Index 16 (97% over 39bp)
CATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCT18400.10436755530346001TruSeq Adapter, Index 16 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)