FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779145
Sequences flagged as poor quality0
Sequence length10-85
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC68450.38473536445877093Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG57470.3230203271796284TruSeq Adapter, Index 7 (97% over 36bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT50260.2824952435017944TruSeq Adapter, Index 16 (100% over 37bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCA40750.22904260192395787TruSeq Adapter, Index 16 (97% over 39bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC38610.21701435239960767TruSeq Adapter, Index 16 (100% over 39bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC26110.14675588555176786TruSeq Adapter, Index 5 (97% over 35bp)
GCTTGCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG25630.14405796042481078TruSeq Adapter, Index 16 (97% over 39bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC21600.12140663071306722TruSeq Adapter, Index 16 (100% over 38bp)
CATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCT21470.12067594265784969TruSeq Adapter, Index 16 (97% over 39bp)
CATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCAT19920.11196389276871756TruSeq Adapter, Index 16 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)