FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758998
Sequences flagged as poor quality0
Sequence length10-85
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCCATTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC70990.40358203932011294Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTGCCATTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG57890.32910782161207686TruSeq Adapter, Index 7 (97% over 36bp)
GCTTGCCATTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT53400.3035819256190172TruSeq Adapter, Index 16 (100% over 37bp)
CATTACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCA42760.24309294268668866TruSeq Adapter, Index 16 (97% over 39bp)
GCTTGCCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC41240.23445165941064172TruSeq Adapter, Index 16 (100% over 39bp)
GCTTGCCATTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC26810.1524163188360646TruSeq Adapter, Index 5 (97% over 35bp)
GCTTGCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG24470.13911329063478184TruSeq Adapter, Index 16 (97% over 39bp)
CATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCT22720.12916444475775413TruSeq Adapter, Index 16 (97% over 39bp)
GCTTGCCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC21100.1199546560030199TruSeq Adapter, Index 16 (100% over 38bp)
CATTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCAT19660.1117681771099228TruSeq Adapter, Index 16 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)