FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758516
Sequences flagged as poor quality0
Sequence length10-85
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC73980.26818767772236957TruSeq Adapter, Index 7 (100% over 39bp)
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA69150.25067826324008996TruSeq Adapter, Index 7 (100% over 37bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA55600.20155764911278384TruSeq Adapter, Index 7 (100% over 40bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC54790.19862128767786738Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG53730.19477864184945817TruSeq Adapter, Index 7 (100% over 36bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT44330.1607023486541314TruSeq Adapter, Index 7 (100% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)