FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2805835
Sequences flagged as poor quality0
Sequence length10-85
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC76890.27403607125864493TruSeq Adapter, Index 7 (100% over 39bp)
GGTCTTGGTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA73010.26020774564434473TruSeq Adapter, Index 7 (100% over 37bp)
GGTCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA57020.20321936250706119TruSeq Adapter, Index 7 (100% over 40bp)
GGTCTTGGTTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC56340.20079584152311167Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTCTTGGTTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG55410.19748132017741601TruSeq Adapter, Index 7 (100% over 36bp)
GGTCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT45910.1636233064310624TruSeq Adapter, Index 7 (100% over 38bp)
GGTCTTGGTTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC28250.1006830408773146Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)