FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005714954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005714954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2878221
Sequences flagged as poor quality0
Sequence length10-85
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCTTGGTTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC137600.4780730875078738Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC71660.24897323728789414TruSeq Adapter, Index 16 (100% over 39bp)
GAGGCTTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC71460.24827836361419084TruSeq Adapter, Index 16 (100% over 38bp)
GAGGCTTGGTTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG64100.22270701242190924TruSeq Adapter, Index 7 (97% over 36bp)
GAGGCTTGGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT56220.195328989677999TruSeq Adapter, Index 16 (100% over 37bp)
GAGGCTTGGTTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC48470.16840263482199594Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGCTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCG41150.14297025836445498TruSeq Adapter, Index 16 (97% over 39bp)
GAGGCTTGGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC30180.10485643736182872TruSeq Adapter, Index 5 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)